Liver bioprinting within a novel support medium with functionalized spheroids, hepatic vein structures, and enhanced post-transplantation vascularization

Jiang, Z; Jin, B; Liang, Z; Wang, Y; Ren, S; Huang, Y; Li, C; Sun, H; Li, Y; Liu, L; Li, N; Wang, J; Cui, Z; Huang, P; Yang, H; Mao, Y; Ye, H

Journal article

Liver bioprinting within a novel support medium with functionalized spheroids, hepatic vein structures, and enhanced post-transplantation vascularization

Abstract:: Cell-laden bioprinting is a promising biofabrication strategy for regenerating bioactive transplants to address organ donor shortages. However, there has been little success in reproducing transplantable artificial organs with multiple distinctive cell types and physiologically relevant architecture. In this study, an omnidirectional printing embedded network (OPEN) is presented as a support medium for embedded 3D printing. The medium is state-of-the-art due to its one-step preparation, fast removal, and versatile ink compatibility. To test the feasibility of OPEN, exceptional primary mouse hepatocytes (PMHs) and endothelial cell line-C166, were used to print hepatospheroid-encapsulated-artificial livers (HEALs) with vein structures following predesigned anatomy-based printing paths in OPEN. PMHs self-organized into hepatocyte spheroids within the ink matrix, whereas the entire cross-linked structure remained intact for a minimum of ten days of cultivation. Cultivated HEALs maintained mature hepatic functions and marker gene expression at a higher level than conventional 2D and 3D conditions in vitro. HEALs with C166-laden vein structures promoted endogenous neovascularization in vivo compared with hepatospheroid-only liver prints within two weeks of transplantation. Collectively, the proposed platform enables the manufacture of bioactive tissues or organs resembling anatomical architecture, and has broad implications for liver function replacement in clinical applications.

Publication status:: Published

Peer review status:: Peer reviewed

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Cite

Cite this record

APA Style

Jiang, Z., Jin, B., Liang, Z., Wang, Y., Ren, S., Huang, Y., Li, C., Sun, H., Li, Y., Liu, L., Li, N., Wang, J., Cui, Z., Huang, P., Yang, H., Mao, Y., & Ye, H. (2024). Liver bioprinting within a novel support medium with functionalized spheroids, hepatic vein structures, and enhanced post-transplantation vascularization. Biomaterials, 311.

MLA Style

Jiang, Z., et al. “Liver Bioprinting within a Novel Support Medium with Functionalized Spheroids, Hepatic Vein Structures, and Enhanced Post-Transplantation Vascularization.” Biomaterials, vol. 311, Elsevier, 2024.

Chicago Style

Jiang, Z, B Jin, Z Liang, Y Wang, S Ren, Y Huang, C Li, et al. 2024. “Liver Bioprinting within a Novel Support Medium with Functionalized Spheroids, Hepatic Vein Structures, and Enhanced Post-Transplantation Vascularization.” Biomaterials 311.
Share
Print