Journal article
Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density
- Abstract:
- Regulation of plasma membrane curvature and composition governs essential cellular processes. The material property of bending rigidity describes the energetic cost of membrane deformations and depends on the plasma membrane molecular composition. Because of compositional fluctuations and active processes, it is challenging to measure it in intact cells. Here, we study the plasma membrane using giant plasma membrane vesicles (GPMVs), which largely preserve the plasma membrane lipidome and proteome. We show that the bending rigidity of plasma membranes under varied conditions is correlated to readout from environment-sensitive dyes, which are indicative of membrane order and microviscosity. This correlation holds across different cell lines, upon cholesterol depletion or enrichment of the plasma membrane, and variations in cell density. Thus, polarity- and viscosity-sensitive probes represent a promising indicator of membrane mechanical properties. Additionally, our results allow for identifying synthetic membranes with a few well defined lipids as optimal plasma membrane mimetics.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 817.1KB, Terms of use)
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- Publisher copy:
- 10.1038/s42003-019-0583-3
Authors
- Publisher:
- Nature Research
- Journal:
- Communications Biology More from this journal
- Volume:
- 2
- Issue:
- 1
- Article number:
- 337
- Publication date:
- 2019-09-13
- Acceptance date:
- 2019-08-15
- DOI:
- EISSN:
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2399-3642
- Pmid:
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31531398
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:1054697
- UUID:
-
uuid:57fc70a9-46f9-4209-a95c-d42e88e8ef51
- Local pid:
-
pubs:1054697
- Source identifiers:
-
1054697
- Deposit date:
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2019-11-08
Terms of use
- Copyright holder:
- Steinkühler et al
- Copyright date:
- 2019
- Notes:
- Copyright © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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