High-throughput cloning, expression, and purification

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High-throughput sequencing of eukaryotic, viral, and bacterial genomes provides a huge database of proteins with potential for structure-function analysis. In response to this opportunity, structural genomics projects have been initiated world-wide with the aim of establishing high-throughput structure determination on a genome-wide scale. Crucial to this effort has been the development of protein production technologies for the highthroughput cloning, expression, and purification of proteins...

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Authors: Owens, R; Nettleship, J; Berrow, N; Sainsbury, S; Aricescu, A

• Publisher: Oxford University Press
• Host title: Macromolecular Crystallography: Conventional and high-throughput methods
• Volume: 9780198520979
• Publication date: 2007-09-01
• DOI: 10.1093/acprof:oso/9780198520979.003.0002
• ISBN: 9780198520979
• Keywords: Expression; Structural proteomics; Purification; Cloning; Genome sequencing