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CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.

Abstract:

The prokaryotic CRISPR/Cas immune system is based on genomic loci that contain incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules, these sequences act as a memory to reject returning invaders. Both the Cascade ribonucleoprotein complex and the Cas3 nuclease/helicase are required for CRISPR interference in Escherichia coli, but it is unknown how natural target DNA molecules are recognized and neutralized by their combined action. Here we show that Cascade eff...

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Journal:
Molecular cell
Volume:
46
Issue:
5
Pages:
595-605
Publication date:
2012-06-05
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
URN:
uuid:5762517d-aea5-478f-9426-df3a10eceb60
Source identifiers:
382763
Local pid:
pubs:382763

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