Journal article
The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases
- Abstract:
- Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2-deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound 'Heatin', containing 1-iminomethyl-2-naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin-mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1-subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin-mediated hypocotyl elongation requires the NITRILASE1-subfamily members, NIT1 and NIT2. Heatin inhibits NIT1-subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1-subfamily substrate indole-3-acetonitrile in vivo. However, levels of the NIT1-subfamily product, bioactive auxin (indole-3-acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1-subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole-3-acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Supplementary materials, pdf, 1.4MB, Terms of use)
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(Preview, Version of record, pdf, 1.2MB, Terms of use)
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- Publisher copy:
- 10.1111/tpj.15250
Authors
+ European Research Council
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- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 616449
+ Deutsche Forschungsgemeinschaft
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- Funder identifier:
- https://ror.org/018mejw64
- Grant:
- INST 20876/127-1 FUGG
+ Biotechnology and Biological Sciences Research Council
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- Funder identifier:
- https://ror.org/00cwqg982
- Grant:
- BB/R017913/1
- BB/S003193/1
+ Ministry of Education Youth and Sports of the Czech Republic
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- Grant:
- CZ.02.1.01/0.0/0.0/16_019/0000827
- Publisher:
- Wiley
- Journal:
- The Plant Journal More from this journal
- Volume:
- 106
- Issue:
- 6
- Pages:
- 1523-1540
- Place of publication:
- England
- Publication date:
- 2021-05-06
- Acceptance date:
- 2021-03-22
- DOI:
- EISSN:
-
1365-313X
- ISSN:
-
0960-7412
- Pmid:
-
33768644
- Language:
-
English
- Keywords:
- Pubs id:
-
1170003
- Local pid:
-
pubs:1170003
- Deposit date:
-
2025-04-29
- ARK identifier:
Terms of use
- Copyright holder:
- van der Woude et al.
- Copyright date:
- 2021
- Rights statement:
- © 2021 The Authors.The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.This is an open access article under the terms of the Creative Commons Attribution License,which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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