Crystal structure of the C1 domain of cardiac myosin binding protein-C: implications for hypertrophic cardiomyopathy.

Journal article

C-protein is a major component of skeletal and cardiac muscle thick filaments. Mutations in the gene encoding cardiac C-protein [cardiac myosin binding protein-C (cMyBP-C)] are one of the principal causes of hypertrophic cardiomyopathy. cMyBP-C is a string of globular domains including eight immunoglobulin-like and three fibronectin-like domains termed C0-C10. It binds to myosin and titin, and probably to actin, and may have both a structural and a regulatory role in muscle function. To help to understand the pathology of the known mutations, we have solved the structure of the immunoglobulin-like C1 domain of MyBP-C by X-ray crystallography to a resolution of 1.55 A. Mutations associated with hypertrophic cardiomyopathy are clustered at one end towards the C-terminus, close to the important C1C2 linker, where they alter the structural integrity of this region and its interactions.

Authors: Govada, L; Carpenter, L; da Fonseca, P; Helliwell, JR; Rizkallah, P

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 378
• Issue: 2
• Pages: 387-397
• Publication date: 2008-04-01
• DOI: 10.1016/j.jmb.2008.02.044
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Cardiomyopathy, Hypertrophic; Molecular Sequence Data; Humans; Carrier Proteins; Crystallography, X-Ray; Protein Structure, Tertiary; Mutation; Protein Folding; Amino Acid Sequence