Kupffer cell staining by an HFE-specific monoclonal antibody: implications for hereditary haemochromatosis.

Journal article

Hereditary haemochromatosis is an inherited disorder of iron absorption that leads to excessive iron storage in the liver and other organs. A candidate disease gene HFE has been identified that encodes a novel MHC class I like protein. We report the development of a monoclonal antibody (HFE-JB1) specific for recombinant refolded HFE protein. The antibody immunoprecipitates a 49 kD protein from the cell line U937, a histiocytic lymphoma. It binds HFE but does not recognize other recombinant no...

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Authors: Bastin, J; Jones, M; O'Callaghan, C; Schimanski, L; Mason, D

• Journal: British journal of haematology
• Volume: 103
• Issue: 4
• Pages: 931-941
• Publication date: 1998-12-01
• DOI: 10.1046/j.1365-2141.1998.01102.x
• EISSN: 1365-2141
• ISSN: 0007-1048
• Language: English
• Keywords: Staining and Labeling; Kupffer Cells; Animals; Liver; Intestines; Antibodies, Monoclonal; Female; Cell Line; Mice, Inbred BALB C; Mice; U937 Cells; Brain; Humans; Placenta; Hemochromatosis; Genes, MHC Class I