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Structural analysis using SHALiPE to reveal RNA G-quadruplex formation in human precursor MicroRNA.

Abstract:

RNA G-quadruplex (rG4) structures are of fundamental importance to biology. A novel approach is introduced to detect and structurally map rG4s at single-nucleotide resolution in RNAs. The approach, denoted SHALiPE, couples selective 2'-hydroxyl acylation with lithium ion-based primer extension, and identifies characteristic structural fingerprints for rG4 mapping. We apply SHALiPE to interrogate the human precursor microRNA 149, and reveal the formation of an rG4 structure in this non-coding ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1002/anie.201603562

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM, RDM Clinical Laboratory Sciences
Balasubramanian, S More by this author
Croucher Foundation More from this funder
Herchel Smith Fellowship More from this funder
Publisher:
Wiley Publisher's website
Journal:
Angewandte Chemie International Edition Journal website
Volume:
55
Issue:
31
Pages:
8958-8961
Publication date:
2016-06-05
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
Pubs id:
pubs:729664
URN:
uri:5573067b-e2d0-444c-8820-4f2f23884e10
UUID:
uuid:5573067b-e2d0-444c-8820-4f2f23884e10
Local pid:
pubs:729664

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