Journal article
Structural analysis using SHALiPE to reveal RNA G-quadruplex formation in human precursor MicroRNA.
- Abstract:
- RNA G-quadruplex (rG4) structures are of fundamental importance to biology. A novel approach is introduced to detect and structurally map rG4s at single-nucleotide resolution in RNAs. The approach, denoted SHALiPE, couples selective 2'-hydroxyl acylation with lithium ion-based primer extension, and identifies characteristic structural fingerprints for rG4 mapping. We apply SHALiPE to interrogate the human precursor microRNA 149, and reveal the formation of an rG4 structure in this non-coding RNA. Additional analyses support the SHALiPE results and uncover that this rG4 has a parallel topology, is thermally stable, and is conserved in mammals. An in vitro Dicer assay shows that this rG4 inhibits Dicer processing, supporting the potential role of rG4 structures in microRNA maturation and post-transcriptional regulation of mRNAs.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.7MB, Terms of use)
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- Publisher copy:
- 10.1002/anie.201603562
Authors
- Publisher:
- Wiley
- Journal:
- Angewandte Chemie International Edition More from this journal
- Volume:
- 55
- Issue:
- 31
- Pages:
- 8958-8961
- Publication date:
- 2016-06-01
- DOI:
- EISSN:
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1521-3773
- ISSN:
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1433-7851
- Pmid:
-
27355429
- Language:
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English
- Keywords:
- Pubs id:
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pubs:729664
- UUID:
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uuid:5573067b-e2d0-444c-8820-4f2f23884e10
- Local pid:
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pubs:729664
- Source identifiers:
-
729664
- Deposit date:
-
2017-11-10
Terms of use
- Copyright holder:
- Sahakyan et al
- Copyright date:
- 2016
- Notes:
- © 2016 The Authors. Published by Wiley-VCH Verlag GmbH and Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
- Licence:
- CC Attribution (CC BY)
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