Genome replication follows a defined temporal programme that can change during cellular differentiation and disease onset. DNA replication results in an increase in DNA copy number that can be measured by high-throughput sequencing. Here we present a protocol to determine genome replication dynamics using DNA copy-number measurements. Cell populations can be obtained in three variants of the method. First, sort-seq reveals the average replication dynamics across S phase in an unperturbed cell...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
- Copyright holder:
- Batrakou et al.
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- Copyright © 2020 The Author(s).
This is the accepted manuscript version of the article. The final version is available from Nature Research at https://doi.org/10.1038/s41596-019-0287-7
DNA copy-number measurement of genome replication dynamics by high-throughput sequencing: the sort-seq, sync-seq and MFA-seq family.
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