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DNA copy-number measurement of genome replication dynamics by high-throughput sequencing: the sort-seq, sync-seq and MFA-seq family.

Abstract:

Genome replication follows a defined temporal programme that can change during cellular differentiation and disease onset. DNA replication results in an increase in DNA copy number that can be measured by high-throughput sequencing. Here we present a protocol to determine genome replication dynamics using DNA copy-number measurements. Cell populations can be obtained in three variants of the method. First, sort-seq reveals the average replication dynamics across S phase in an unperturbed cell...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41596-019-0287-7

Authors


Batrakou, DG More by this author
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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
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ORCID:
0000-0003-3865-6729
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ORCID:
0000-0003-2001-076X
Publisher:
Nature Research Publisher's website
Journal:
Nature Protocols Journal website
Volume:
15
Issue:
3
Pages:
1255-1284
Publication date:
2020-02-12
Acceptance date:
2019-12-16
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pmid:
32051615
Pubs id:
1087953
Local pid:
pubs:1087953

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