Journal article

Gel-aided sample preparation (GASP) - a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells

Abstract:: We describe a "gel-assisted" proteomic sample preparation method for mass spectrometry analysis. Solubilized protein extracts or intact cells are co-polymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation and matrix formation. Gel-aided sample preparation (GASP) has been optimized to be highly flexible, scalable and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types and accessible to non-specialists.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Fischer, R., & Kessler, B. (2015). Gel-aided sample preparation (GASP) - a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells. Proteomics, 15(7), 1224–1229.

MLA Style

Fischer, R., and B. Kessler. “Gel-Aided Sample Preparation (GASP) - a Simplified Method for Gel-Assisted Proteomic Sample Generation from Protein Extracts and Intact Cells.” Proteomics, vol. 15, no. 7, Wiley, 2015, pp. 1224–29.

Chicago Style

Fischer, R, and B Kessler. 2015. “Gel-Aided Sample Preparation (GASP) - a Simplified Method for Gel-Assisted Proteomic Sample Generation from Protein Extracts and Intact Cells.” Proteomics 15 (7): 1224–29.
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Files:: Fischer_et_al-2015-PROTEOMICS.pdf

(Preview, Version of record, pdf, 452.7KB, Terms of use)

Publisher copy:: 10.1002/pmic.201400436

Authors

+ Fischer, R More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author

+ Kessler, B More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author

Publisher:: Wiley
Journal:: Proteomics More from this journal
Volume:: 15
Issue:: 7
Pages:: 1224–1229
Publication date:: 2015-02-05
Acceptance date:: 2014-12-05
DOI:: 10.1002/pmic.201400436
EISSN:: 1615-9861
ISSN:: 1615-9861

Language:: English
Keywords:: proteome

humans

proteomics

spectrometry, mass, matrix-assisted laser desorption-ionization

cell extracts

cells, cultured

SBTMR

acrylic resins
Pubs id:: pubs:499840
UUID:: uuid:5546d825-5143-4c5d-a428-96c5541680f0
Local pid:: pubs:499840
Source identifiers:: 499840
Deposit date:: 2014-12-26

Terms of use

Copyright holder:: Roman Fischer and Benedikt Kessler
Copyright date:: 2015
Notes:: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Licence:: CC Attribution (CC BY)

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