Journal article
The impact of viral mutations on recognition by SARS-CoV-2 specific T-cells
- Abstract:
- We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 2.0MB, Terms of use)
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- Publisher copy:
- 10.1016/j.isci.2021.103353
Authors
- Publisher:
- Cell Press
- Journal:
- iScience More from this journal
- Volume:
- 24
- Issue:
- 11
- Article number:
- 103353
- Publication date:
- 2021-11-19
- Acceptance date:
- 2021-10-22
- DOI:
- ISSN:
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2589-0042
- Language:
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English
- Keywords:
- Pubs id:
-
1206227
- Local pid:
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pubs:1206227
- Deposit date:
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2021-11-01
Terms of use
- Copyright holder:
- de Silva et al.
- Copyright date:
- 2021
- Rights statement:
- ©2021 The Author(s). This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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