Journal article
Synthetic chemical inducers and genetic decoupling enable orthogonal control of the rhaBAD promoter.
- Abstract:
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External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD promoter has properties superior to more commonly-used inducible expression systems, but is marred by transient expression caused by degradation of the native inducer, L-rhamnose. To address this problem, 35 analogs of L-rhamnose were screened for induction of the rhaBAD promoter, but n...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.3MB, Terms of use)
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- Publisher copy:
- 10.1021/acssynbio.6b00030
Authors
Funding
+ Biotechnology and Biological Sciences Research Council
More from this funder
Funding agency for:
Heap, J
Grant:
BB/M011321/1
+ Leverhulme Trust
More from this funder
Funding agency for:
Fleet, G
Grant:
Emeritus Leverhulme Research Fellowship
Bibliographic Details
- Publisher:
- American Chemical Society
- Journal:
- ACS Synthetic Biology More from this journal
- Volume:
- 5
- Issue:
- 10
- Pages:
- 1136–1145
- Publication date:
- 2016-05-31
- Acceptance date:
- 2016-05-31
- DOI:
- EISSN:
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2161-5063
Item Description
- Keywords:
- Pubs id:
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pubs:625059
- UUID:
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uuid:54a0b8ba-b79f-40b2-b86e-0558c1390700
- Local pid:
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pubs:625059
- Source identifiers:
-
625059
- Deposit date:
-
2016-06-02
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2016
- Notes:
- Copyright © 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License.
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