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Artificial mirtron-mediated gene knockdown: functional DMPK silencing in mammalian cells.

Abstract:

Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs. Here we present a design algorithm for artificial mirtrons and demonstrate, for the first time, efficient gene knockdown of myotonic dystrophy protein kinase (DMPK) target sequences in Renilla luciferase 3' UTR and subsequently pathogenic DMPK mRNA, causative of Type I myotonic dystrophy, using artificial mirtrons cloned as eGFP introns....

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Publisher copy:
10.1261/rna.030601.111

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
Journal:
RNA (New York, N.Y.)
Volume:
18
Issue:
7
Pages:
1328-1337
Publication date:
2012-07-05
DOI:
EISSN:
1469-9001
ISSN:
1355-8382
URN:
uuid:53b69888-2b05-415c-8a81-a18d42af49b0
Source identifiers:
334765
Local pid:
pubs:334765

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