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Generation of human iPSC-derived cerebellar organoids

Abstract:: Human induced pluripotent stem cell (hiPSC)-derived brain organoids are a novel tool to model human-specific aspects of brain development. Here, we describe a reproducible protocol for generating hiPSC-derived cerebellar organoids that have been experimentally validated to contain typical cerebellar cell populations. Generated three-dimensional cerebellar organoids can be kept in long-term culture or further be maintained in dissociated culture settings to allow for single-cell resolution in downstream applications. The current protocol is an improvement on earlier methods that require co-culture of hiPSC-derived neurons with murine cerebellar cell populations.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Tolonen, J.-P., Apsley, E. J., van Essen, M. J., & Becker, B. E. (2024). Generation of human iPSC-derived cerebellar organoids. In B. Ding & Y. Tang (Eds.), Human Induced Pluripotent Stem Cells (1st ed., pp. 221–238). Springer.

MLA Style

Tolonen, J.-P., et al. “Generation of Human IPSC-Derived Cerebellar Organoids.” Human Induced Pluripotent Stem Cells, edited by B Ding and Y Tang, 1st ed., Springer, 2024, pp. 221–38.

Chicago Style

Tolonen, J-P, EJ Apsley, MJ van Essen, and BE Becker. 2024. “Generation of Human IPSC-Derived Cerebellar Organoids.” In Human Induced Pluripotent Stem Cells, edited by B Ding and Y Tang, 1st ed., 221–38. Neuromethods. Springer.
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Publisher copy:: 10.1007/978-1-0716-3999-3_18

Authors

+ Tolonen, J-P More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Clinical Neurosciences
Role:: Author

+ Apsley, EJ More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Clinical Neurosciences
Role:: Author

+ van Essen, MJ More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Clinical Neurosciences
Role:: Author

+ Becker, BE More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Clinical Neurosciences
Oxford college:: Wadham College
Role:: Author
ORCID:: 0000-0002-5238-4902

Contributors

Role:: Editor

Role:: Editor

+ European Commission More from this funder

Funder identifier:: https://ror.org/00k4n6c32
Grant:: 101023312
Programme:: PEDIATAX

+ Cancer Research UK More from this funder

Funder identifier:: https://ror.org/054225q67
Funding agency for:: van Essen, MJ

+ Biotechnology and Biological Sciences Research Council More from this funder

Funder identifier:: https://ror.org/00cwqg982
Funding agency for:: Apsley, EJ

Publisher:: Springer
Host title:: Human Induced Pluripotent Stem Cells
Pages:: 221-238
Chapter number:: 18
Series:: Neuromethods
Series number:: 210
Place of publication:: New York
Publication date:: 2024-07-14
Edition:: 1
DOI:: 10.1007/978-1-0716-3999-3_18
EISSN:: 1940-6045
ISSN:: 0893-2336
EISBN:: 9781071639993
ISBN:: 9781071639986

Language:: English
Keywords:: cerebellum

personalized medicine

spinocerebellar ataxias

cerebellar development

cerebellar organoids

human induced pluripotent stem cells
Subtype:: Chapter
Pubs id:: 2018912
Local pid:: pubs:2018912
Deposit date:: 2025-01-07

Terms of use

Copyright holder:: Tolonen et al.
Copyright date:: 2024
Rights statement:: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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