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Quadrupole-time-of-flight mass spectrometer modified for higher-energy dissociation reduces protein assemblies to peptide fragments.

Abstract:

The gas-phase dissociation of protein assemblies is becoming crucial for the application of mass spectrometry to structural biology. However certain aspects of the dissociation mechanism remain elusive. Moreover, many protein complexes resist dissociation at the energies accessible with current instrumentation. Here we report new insights into the collision-induced dissociation mechanism of protein assemblies. By holding activation energy constant and varying the charge state of the precursor...

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Publication status:
Published

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Publisher copy:
10.1021/ac801950u

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Ruotolo, BT More by this author
Wildgoose, J More by this author
Gilbert, A More by this author
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Journal:
Analytical chemistry
Volume:
81
Issue:
3
Pages:
1270-1274
Publication date:
2009-02-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:535327de-08b2-4019-a0b4-4bed5149da69
Source identifiers:
59319
Local pid:
pubs:59319

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