Journal article
4D Visualization of replication foci in mammalian cells corresponding to individual replicons
- Abstract:
- Since the pioneering proposal of the replicon model of DNA replication 50 years ago, the predicted replicons have not been identified and quantified at the cellular level. Here, we combine conventional and super-resolution microscopy of replication sites in live and fixed cells with computational image analysis. We complement these data with genome size measurements, comprehensive analysis of S-phase dynamics and quantification of replication fork speed and replicon size in human and mouse cells. These multidimensional analyses demonstrate that replication foci (RFi) in three-dimensional (3D) preserved somatic mammalian cells can be optically resolved down to single replicons throughout S-phase. This challenges the conventional interpretation of nuclear RFi as replication factories, that is, the complex entities that process multiple clustered replicons. Accordingly, 3D genome organization and duplication can be now followed within the chromatin context at the level of individual replicons.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 2.8MB, Terms of use)
-
- Publisher copy:
- 10.1038/ncomms11231
Authors
+ Nanosystems Initiative Munich
More from this funder
- Funding agency for:
- Leonhardt, H
- Grant:
- DFG SFB 1064 A17/Z05
+ German Research Council
More from this funder
- Funding agency for:
- Schermelleh, L
- Leonhardt, H
- Cardoso, M
- Grant:
- DFG SFB-TR5
- DFG SFB 1064 A17/Z05
- DFG CA 198/9
- Publisher:
- Nature Publishing Group
- Journal:
- Nature Communications More from this journal
- Volume:
- 7
- Pages:
- 11231
- Publication date:
- 2016-04-07
- Acceptance date:
- 2016-03-03
- DOI:
- EISSN:
-
2041-1723
- Pubs id:
-
pubs:614778
- UUID:
-
uuid:52fa8365-00c6-4761-9b40-3ad3b608b4b1
- Local pid:
-
pubs:614778
- Source identifiers:
-
614778
- Deposit date:
-
2016-04-10
Terms of use
- Copyright holder:
- © Chagin, et al
- Copyright date:
- 2016
- Notes:
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record