Journal article
Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure
- Abstract:
- The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Publisher copy:
- 10.1371/journal.pbio.1000102
- Publisher:
- Public Library of Science
- Journal:
- PLoS Biology More from this journal
- Volume:
- 7
- Issue:
- 5
- Article number:
- e1000102
- Publication date:
- 2009-05-01
- Edition:
- Publisher's version
- DOI:
- EISSN:
-
1545-7885
- ISSN:
-
1544-9173
- Language:
-
English
- Keywords:
- Subjects:
- UUID:
-
uuid:526dfd47-90c0-4279-a961-04a573f0e71d
- Local pid:
-
ora:2807
- Deposit date:
-
2009-06-04
Terms of use
- Copyright holder:
- Korkhin et al
- Copyright date:
- 2009
- Notes:
- Citation: Korkhin, Y. et al. (2009). 'Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure', PLoS Biology, 7(5), e1000102. [Available at http://biology.plosjournals.org/]. © Korkhin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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