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Journal article

Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy

Abstract:

Objective

Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. However, current modalities for drug testing, such as electrophysiology and fluorescence imaging have inherent drawbacks. To circumvent these problems, we report the development of a bioluminescent Ca2+ indicator GmNL(Ca2+), and its application in a customized microscope for high-throughput drug screening.

Results

GmNL(Ca2...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1186/s13104-018-3421-7

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM Cardiovascular Medicine
Role:
Author
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Publisher:
BioMed Central Publisher's website
Journal:
BMC Research Notes Journal website
Volume:
11
Issue:
1
Pages:
313
Publication date:
2018-05-18
Acceptance date:
2018-05-10
DOI:
EISSN:
1756-0500
Pubs id:
pubs:853069
URN:
uri:51be269e-a1af-41bc-a8f8-9aa468d11ae9
UUID:
uuid:51be269e-a1af-41bc-a8f8-9aa468d11ae9
Local pid:
pubs:853069

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