Journal article
Hypothermic oxygenated machine perfusion of the human donor pancreas
- Abstract:
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Background Transplantation of beta cells by pancreas or islet transplantation is the treatment of choice for a selected group of patients suffering from type 1 diabetes mellitus. Pancreata are frequently not accepted for transplantation, because of the relatively high vulnerability of these organs to ischemic injury. In this study, we evaluated the effects of hypothermic machine perfusion (HMP) on the quality of human pancreas grafts.
Methods Five pancreata derived from donation after circulatory death (DCD) and 5 from donation after brain death (DBD) donors were preserved by oxygenated HMP. Hypothermic machine perfusion was performed for 6 hours at 25 mm Hg by separate perfusion of the mesenteric superior artery and the splenic artery. Results were compared with those of 10 pancreata preserved by static cold storage.
Results During HMP, homogeneous perfusion of the pancreas could be achieved. Adenosine 5′-triphosphate concentration increased 6,8-fold in DCD and 2,6-fold in DBD pancreata. No signs of cellular injury, edema or formation of reactive oxygen species were observed. Islets of Langerhans with good viability and in vitro function could be isolated after HMP.
Conclusions Oxygenated HMP is a feasible and safe preservation method for the human pancreas that increases tissue viability.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.5MB, Terms of use)
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- Publisher copy:
- 10.1097/TXD.0000000000000829
Authors
- Publisher:
- Wolters Kluwer Health
- Journal:
- Transplantation direct More from this journal
- Volume:
- 4
- Issue:
- 10
- Article number:
- e388
- Publication date:
- 2018-10-01
- Acceptance date:
- 2018-07-18
- DOI:
- EISSN:
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2373-8731
- Pmid:
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30498765
- Language:
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English
- Pubs id:
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pubs:952878
- UUID:
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uuid:50f07fa5-447e-493e-9de5-b637489388fb
- Local pid:
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pubs:952878
- Source identifiers:
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952878
- Deposit date:
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2019-08-05
- ARK identifier:
Terms of use
- Copyright holder:
- Leemkuil et al
- Copyright date:
- 2018
- Notes:
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Copyright © 2018 The Author(s). Transplantation Direct. Published by Wolters
Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided
it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal
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