Journal article
Determination of torque generation from the power stroke of Escherichia coli F1-ATPase
- Abstract:
- The torque generated by the power stroke of Escherichia coli F1-ATPase was determined as a function of the load from measurements of the velocity of the γ-subunit obtained using a 0.25 µs time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the γ-subunit was able to rotate with a 20× increase in viscosity, the transition time decreased from 0.4 ms to 5.26 ms. The torque was measured to be 63 ± 8 pN nm, independent of the load on the enzyme.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 434.6KB, Terms of use)
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- Publisher copy:
- 10.1016/j.bbabio.2008.04.016
Authors
- Publisher:
- Elsevier
- Journal:
- Biochimica et Biophysica Acta (BBA) - Bioenergetics More from this journal
- Volume:
- 1777
- Issue:
- 7-8
- Pages:
- 579-582
- Publication date:
- 2008-04-18
- Acceptance date:
- 2008-04-14
- DOI:
- ISSN:
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0006-3002
- Language:
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English
- Keywords:
- Pubs id:
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pubs:155568
- UUID:
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uuid:50dc80ee-1eea-4b02-8d5c-1bafa52e1e80
- Local pid:
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pubs:155568
- Source identifiers:
-
155568
- Deposit date:
-
2013-11-16
Terms of use
- Copyright holder:
- Elsevier B.V.
- Copyright date:
- 2008
- Rights statement:
- © 2008 Elsevier B.V. All rights reserved.
- Notes:
- This is the accepted manuscript version of the article. The final version is available from Elsevier at: 10.1016/j.bbabio.2008.04.016
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