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Determination of torque generation from the power stroke of Escherichia coli F1-ATPase.

Abstract:

The torque generated by the power stroke of Escherichia coli F(1)-ATPase was determined as a function of the load from measurements of the velocity of the gamma-subunit obtained using a 0.25 micros time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the gamma-subunit was able to rotate with a 20x increase in viscosity, the transition time decreased from 0.4 ms to ...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Role:
Author
Journal:
Biochimica et biophysica acta
Volume:
1777
Issue:
7-8
Pages:
579-582
Publication date:
2008-01-01
DOI:
ISSN:
0006-3002
URN:
uuid:50dc80ee-1eea-4b02-8d5c-1bafa52e1e80
Source identifiers:
155568
Local pid:
pubs:155568

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