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The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86

Abstract:
Under pressure from neutralising antibodies induced by vaccination or infection the SARS-CoV-2 spike gene has become a hotspot for evolutionary change, leading to the failure of all mAbs developed for clinical use. Most potent antibodies bind to the receptor binding domain which has become heavily mutated. Here we study responses to a conserved epitope in sub-domain-1 (SD1) of spike which have become more prominent because of mutational escape from antibodies directed to the receptor binding domain. Some SD1 reactive mAbs show potent and broad neutralization of SARS-CoV-2 variants. We structurally map the dominant SD1 epitope and provide a mechanism of action by blocking interaction with ACE2. Mutations in SD1 have not been sustained to date, but one, E554K, leads to escape from mAbs. This mutation has now emerged in several sublineages including BA.2.86, reflecting selection pressure on the virus exerted by the increasing prominence of the anti-SD1 response.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-024-46982-6

Authors

More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
NDM (CAMS)
Role:
Author
ORCID:
0000-0002-7188-5243
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
NDM (CAMS)
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
NDM (CAMS)
Role:
Author
ORCID:
0000-0003-4250-0989
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Centre for Human Genetics
Role:
Author
ORCID:
0000-0002-4850-9709
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
ORCID:
0000-0002-4641-5442


More from this funder
Funder identifier:
https://ror.org/029chgv08
Grant:
093305/Z/10/Z
203141/Z/16/Z
090532/Z/09/Z
More from this funder
Funder identifier:
https://ror.org/03x94j517
Grant:
MR/N00065X/1


Publisher:
Springer Nature
Journal:
Nature Communications More from this journal
Volume:
15
Issue:
1
Article number:
2734
Place of publication:
England
Publication date:
2024-03-28
Acceptance date:
2024-03-15
DOI:
EISSN:
2041-1723
ISSN:
2041-1723
Pmid:
38548763


Language:
English
Pubs id:
1934171
Local pid:
pubs:1934171
Deposit date:
2025-02-11
ARK identifier:

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