Book section
An Innovative Standard Operation Procedure for Isolating GMP-Grade CD4+CD25+ T Cells from Non-Mobilized Leukapheresis
- Abstract:
- This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 271.9KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-4939-8938-6_6
Authors
- Publisher:
- Humana Press
- Host title:
- Immunological Tolerance
- Volume:
- 1899
- Pages:
- 67-83
- Publication date:
- 2019-01-17
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Pmid:
-
30649766
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:965840
- UUID:
-
uuid:4fe1c9c1-a7ad-48d7-8260-9bc910b4334f
- Local pid:
-
pubs:965840
- Source identifiers:
-
965840
- Deposit date:
-
2019-03-21
Terms of use
- Copyright holder:
- Springer Science+Business Media, LLC
- Copyright date:
- 2019
- Notes:
- © Springer Science+Business Media, LLC, part of Springer Nature 2019. This is the accepted manuscript version of the article. The final version is available online from Humana Press at: https://doi.org/10.1007/978-1-4939-8938-6_6
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