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Journal article

Bromodomain-peptide displacement assays for interactome mapping and inhibitor discovery.

Abstract:

Histone lysine acetylation is a key component of epigenetic regulation of gene transcription. Bromodomains, found in histone acetyl transferases and other chromatin-associated proteins, bind selectively to acetylated lysines, acting as "readers" of the histone code, and have recently been shown to contain a druggable binding pocket. Here we report the development of high-throughput assays that quantify the binding of bromodomains to acetylated histone peptides. We have used these assays to sc...

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Publication status:
Published

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Publisher copy:
10.1039/c1mb05099k

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Journal:
Molecular bioSystems
Volume:
7
Issue:
10
Pages:
2899-2908
Publication date:
2011-10-05
DOI:
EISSN:
1742-2051
ISSN:
1742-206X
URN:
uuid:4ed79667-f0f6-4799-960a-850e7ce4fd28
Source identifiers:
177354
Local pid:
pubs:177354

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