Journal article

A microfluidic chip for immobilization and imaging of Ciona intestinalis larvae

Abstract:: Sea squirts (Tunicata) are chordates and develop a swimming larva with a small and defined number of individually identifiable cells. This offers the prospect of connecting specific stimuli to behavioral output and characterizing the neural activity that links these together. Here, we describe the development of a microfluidic chip that allows live larvae of the sea squirt Ciona intestinalis to be immobilized and recorded. By generating transgenic larvae expressing GCaAMP6m in defined cells, we show that calcium ion levels can be recorded from immobilized larvae, while microfluidic control allows larvae to be exposed to specific waterborne stimuli. We trial this on sea water carrying increased levels of carbon dioxide, providing evidence that larvae can sense this gas.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Poncelet, G., Parolini, L., & Shimeld, S. M. (2024). A microfluidic chip for immobilization and imaging of Ciona intestinalis larvae. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 342(7), 443–452.

MLA Style

Poncelet, G., et al. “A Microfluidic Chip for Immobilization and Imaging of Ciona Intestinalis Larvae.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, vol. 342, no. 7, Wiley, 2024, pp. 443–52.

Chicago Style

Poncelet, G, L Parolini, and SM Shimeld. 2024. “A Microfluidic Chip for Immobilization and Imaging of Ciona Intestinalis Larvae.” Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 342 (7): 443–52.
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Files:: Poncelet_et_al_2024_A_microfluidic_chip.pdf

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Publisher copy:: 10.1002/jez.b.23267

Authors

+ Poncelet, G More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Biology
Role:: Author

+ Parolini, L More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: NDM Experimental Medicine
Role:: Author

+ Shimeld, SM More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Biology
Oxford college:: Balliol College
Role:: Author
ORCID:: 0000-0003-0195-7536

+ John Fell Fund More from this funder

Publisher:: Wiley
Journal:: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution More from this journal
Volume:: 342
Issue:: 7
Pages:: 443-452
Publication date:: 2024-06-07
Acceptance date:: 2024-05-21
DOI:: 10.1002/jez.b.23267
EISSN:: 1552-5015
ISSN:: 1552-5007

Language:: English
Keywords:: carbon dioxide

neuron

Ciona

olfaction

microfluidic
Pubs id:: 1998728
Local pid:: pubs:1998728
Deposit date:: 2024-05-25

Terms of use

Copyright holder:: Poncelet et al.
Copyright date:: 2024
Rights statement:: © 2024 The Author(s). Journal of Experimental Zoology Part B: Molecular and Developmental Evolution published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Licence:: CC Attribution (CC BY)

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