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Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

Abstract:

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straigh...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1038/s41592-018-0085-0

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Role:
Author
ORCID:
0000-0002-3710-5602
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Publisher:
Springer Nature Publishing Group Publisher's website
Journal:
Nature Methods Journal website
Volume:
15
Pages:
669-676
Publication date:
2018-08-31
Acceptance date:
2018-05-25
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pubs id:
pubs:912075
URN:
uri:4e671113-967a-4fbd-bb06-dfcf381cfd8e
UUID:
uuid:4e671113-967a-4fbd-bb06-dfcf381cfd8e
Local pid:
pubs:912075

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