- Abstract:
-
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straigh...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's Version
- Files:
-
-
(pdf, 497.6KB)
-
(pdf, 4.1MB)
-
- Publisher copy:
- 10.1038/s41592-018-0085-0
-
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- Publisher:
- Springer Nature Publishing Group Publisher's website
- Journal:
- Nature Methods Journal website
- Volume:
- 15
- Pages:
- 669-676
- Publication date:
- 2018-08-31
- Acceptance date:
- 2018-05-25
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- Pubs id:
-
pubs:912075
- URN:
-
uri:4e671113-967a-4fbd-bb06-dfcf381cfd8e
- UUID:
-
uuid:4e671113-967a-4fbd-bb06-dfcf381cfd8e
- Local pid:
- pubs:912075
- Language:
- English
- Keywords:
- Copyright holder:
- Hellenkamp et al.
- Copyright date:
- 2018
- Notes:
-
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Note: an erratum exists for this article, originally published and available at: https://doi.org/10.1038/s41592-018-0193-x
Journal article
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
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