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Possible pitfalls in the identification of glycophorin-binding proteins of Plasmodium falciparum.

Abstract:

Plasmodium falciparum proteins that bind to the putative erythrocyte receptor (glycophorin) have been identified in several laboratories by their ability to bind to glycophorin immobilized on aminoethyl-BioGel (AE-BioGel). We here report that several parasite proteins bind to AE-BioGel in the absence of coupled glycophorin. Binding is apparently due to the strong ion-exchange properties of the matrix, and is sensitive to ionic conditions such as the degree of equilibration of the matrix and t...

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Publication status:
Published

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Publisher copy:
10.1084/jem.166.2.376

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
Journal:
The Journal of experimental medicine
Volume:
166
Issue:
2
Pages:
376-390
Publication date:
1987-08-05
DOI:
EISSN:
1540-9538
ISSN:
0022-1007
URN:
uuid:4e0dc6f5-fa2c-46a4-80d4-21c1d5293444
Source identifiers:
4753
Local pid:
pubs:4753

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