Journal article icon

Journal article

emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging

Abstract:

Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo-electron microscopy (cryo-EM). Such complexes can be studied by cryo-electron tomography (cryo-ET) combined with subtomogram alignment and classification, which in exceptional cases achieves subnanometer resolution, yielding insight into structure-function relationships. However, it remains challenging to apply this approach to specimens that exhibit conforma...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

Actions


Access Document


Files:
Publisher copy:
10.1038/s41592-018-0167-z

Authors


More by this author
Role:
Author
ORCID:
0000-0001-7777-0298
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Structural Biology
Role:
Author
ORCID:
0000-0003-1803-691X
More from this funder
Funding agency for:
Zhang, P
Publisher:
Springer Nature Publisher's website
Journal:
Nature methods Journal website
Volume:
15
Pages:
955–961
Publication date:
2018-10-22
Acceptance date:
2018-07-25
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pubs id:
pubs:935413
URN:
uri:4dcfb499-f5e6-45bf-85c7-e02cebb37652
UUID:
uuid:4dcfb499-f5e6-45bf-85c7-e02cebb37652
Local pid:
pubs:935413
Language:
English
Keywords:

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP