Using cAMP sensors to study cardiac nanodomains

Journal article

3',5'-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform: depending on the stimulus, cAMP signals of different amplitudes and kinetics are generated in different subcellular compartments, eliciting defined physiological effects. In this review, we focus on how real-time imaging using fluorescence resonance energy transfer (FRET)-based reporters has provided mechanistic insight into the compartmentalisation of the cAMP signalling pathway and allowed for the precise definition of the regulation and function of subcellular cAMP nanodomains.

Authors: Schleicher, K; Zaccolo, M

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: MDPI
• Journal: Journal of Cardiovascular Development and Disease
• Volume: 5
• Issue: 1
• Article number: 17
• Publication date: 2018-03-13
• Acceptance date: 2018-03-09
• DOI: 10.3390/jcdd5010017
• ISSN: 2308-3425
• Pmid: 29533995
• Language: English
• Keywords: cardiac biology; protein kinase A; A kinase anchoring proteins; phosphodiesterases; signalling; fluorescence resonance energy transfer; real-time imaging; compartmentalisation; 3′,5′-cyclic adenosine monophosphate