Journal article
Defensive mechanism in human cholangiocarcinoma cells against hypoxia by cyanidin-3-glucoside treatment
- Abstract:
- Abstract: Background and Objective: Cyanidin-3-glucoside (C3G) is one of the major bioactive compounds in anthocyanins, which have been shown to prevent a large spectrum of diseases. It has been reported that hypoxia is a common characteristic of many types of solid tumor and that the hypoxia-inducible factor 1α (HIF-1α) plays an important role in the process of angiogenesis and metastasis in cancer cells. The objective of this study was to explore the effects of C3G on HIF-1α activation in cholangiocarcinoma (CCA) cells. Materials and Methods: The human cholangiocarcinoma cell line QBC939 was used in this study. Hypoxic conditions were achieved by using the chemical hypoxia agent cobalt chloride or incubation at 1% O2. and MTT assay, Luciferase reporter assay, flow cytometric apoptosis assay, western blot, RT-PCR and immuno fluorescence methods were used to determine the C3G efficacy. Results: C3G markedly decreased the hypoxia-induced accumulation of HIF-1α reporter activity and protein expression in a dose-dependent manner and exerted a large apoptotic effect in CCA cells. Furthermore, C3G reduced expression of several genes downstream of HIF-1α, such as vascular endothelial growth factor and erythropoietin, without affecting the expression level of HIF-1α mRNA, thus indicating that C3G acts as an inhibitor of HIF-1α mRNA translation. Conclusion: This study provides a novel strategy to develop a low toxicity, high efficiency and multi-target natural active substance for the treatment of cancers.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 1.1MB, Terms of use)
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- Publisher copy:
- 10.3923/ijp.2019.361.369
Authors
- Publisher:
- Asian Network for Scientific Information
- Journal:
- International Journal of Pharmacology More from this journal
- Volume:
- 15
- Issue:
- 3
- Pages:
- 361-369
- Publication date:
- 2019-03-15
- Acceptance date:
- 2018-12-13
- DOI:
- EISSN:
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1812-5700
- ISSN:
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1811-7775
- Keywords:
- Pubs id:
-
991282
- Local pid:
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pubs:991282
- Deposit date:
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2020-03-11
Terms of use
- Copyright holder:
- Guang-Hua Xie et al.
- Copyright date:
- 2019
- Rights statement:
- Copyright: © 2019 Guang-Hua Xie et al. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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