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Negative Ions Enhance Survival of Membrane Protein Complexes

Abstract:

Membrane protein complexes are commonly introduced to the mass spectrometer solubilized in detergent micelles. The collisional activation used to remove the detergent, however, often causes protein unfolding and dissociation. As in the case for soluble proteins, electrospray in the positive ion mode is most commonly used for the study of membrane proteins. Here we show several distinct advantages of employing the negative ion mode. Negative polarity can yield lower average charge states for m...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1007/s13361-016-1381-5

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Department:
Oxford, MPLS, Chemistry, Physical & Theoretical Chem
Role:
Author
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Department:
Oxford, MPLS, Chemistry, Physical & Theoretical Chem
Role:
Author
Publisher:
Springer Verlag Publisher's website
Journal:
Journal of the American Society for Mass Spectrometry Journal website
Issue:
6
Publication date:
2016-04-22
Acceptance date:
2016-03-08
DOI:
EISSN:
1879-1123
ISSN:
1044-0305
Pubs id:
pubs:624863
URN:
uri:4ce3ed7b-0afe-4a93-9b3c-e0aeda76d668
UUID:
uuid:4ce3ed7b-0afe-4a93-9b3c-e0aeda76d668
Local pid:
pubs:624863

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