- Abstract:
-
Membrane protein complexes are commonly introduced to the mass spectrometer solubilized in detergent micelles. The collisional activation used to remove the detergent, however, often causes protein unfolding and dissociation. As in the case for soluble proteins, electrospray in the positive ion mode is most commonly used for the study of membrane proteins. Here we show several distinct advantages of employing the negative ion mode. Negative polarity can yield lower average charge states for m...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.5MB)
-
(pdf, 206.6KB)
-
- Publisher copy:
- 10.1007/s13361-016-1381-5
-
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- Publisher:
- Springer Verlag Publisher's website
- Journal:
- Journal of the American Society for Mass Spectrometry Journal website
- Issue:
- 6
- Publication date:
- 2016-04-22
- Acceptance date:
- 2016-03-08
- DOI:
- EISSN:
-
1879-1123
- ISSN:
-
1044-0305
- Pubs id:
-
pubs:624863
- URN:
-
uri:4ce3ed7b-0afe-4a93-9b3c-e0aeda76d668
- UUID:
-
uuid:4ce3ed7b-0afe-4a93-9b3c-e0aeda76d668
- Local pid:
- pubs:624863
- Language:
- English
- Keywords:
- Copyright holder:
- Liko et al.
- Copyright date:
- 2016
- Notes:
- © The Author(s), 2016. This article is published with open access at Springerlink.com.
Journal article
Negative Ions Enhance Survival of Membrane Protein Complexes
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