Journal article
Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors
- Abstract:
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Purpose: Aging is associated with changes in muscle energy metabolism. Proton (1H) and phosphorous (31P) magnetic resonance spectroscopy (MRS) has been successfully applied for non-invasive investigation of skeletal muscle metabolism. The aim of this study was to detect differences in adenosine triphosphate (ATP) production in the aging muscle by 31P-MRS and to identify potential changes associated with buffer capacity of muscle carnosine by 1H-MRS.
Methods: Fifteen young and nineteen elderly volunteers were examined. 1H and 31P-MRS spectra were acquired at high field (7T). The investigation included carnosine quantification using 1H-MRS and resting and dynamic 31P-MRS, both including saturation transfer measurements of phosphocreatine (PCr), and inorganic phosphate (Pi)-to-ATP metabolic fluxes.
Results: Elderly volunteers had higher time constant of PCr recovery (τPCr) in comparison to the young volunteers. Exercise was connected with significant decrease in PCr-to-ATP flux in both groups. Moreover, PCr-to-ATP flux was significantly higher in young compared to elderly both at rest and during exercise. Similarly, an increment of Pi-to-ATP flux with exercise was found in both groups but the intergroup difference was only observed during exercise. Elderly had lower muscle carnosine concentration and lower postexercise pH. A strong increase in phosphomonoester (PME) concentration was observed with exercise in elderly, and a faster Pi:PCr kinetics was found in young volunteers compared to elderly during the recovery period.
Conclusion: Observations of a massive increment of PME concentration together with high Pi-to-ATP flux during exercise in seniors refer to decreased ability of the muscle to meet the metabolic requirements of exercise and thus a limited ability of seniors to effectively support the exercise load.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 1.8MB, Terms of use)
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- Publisher copy:
- 10.3389/fphys.2020.00644
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Physiology More from this journal
- Volume:
- 11
- Article number:
- 644
- Publication date:
- 2020-06-29
- Acceptance date:
- 2020-05-20
- DOI:
- EISSN:
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1664-042X
- Language:
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English
- Keywords:
- Pubs id:
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1117245
- Local pid:
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pubs:1117245
- Deposit date:
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2020-07-09
Terms of use
- Copyright holder:
- Krumpolec et al.
- Copyright date:
- 2020
- Rights statement:
- Copyright © 2020 Krumpolec, Klepochová, Just, Tušek Jelenc, Frollo, Ukropec, Ukropcová, Trattnig, Krššák and Valkovič. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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