In vivo NMR analysis of tropane alkaloid metabolism in transformed root and de-differentiated cultures of Datura stramonium

Journal article

In vivo 15N NMR spectroscopy has been used to study the metabolism of [15N]tropianone is transformed root cultures of Datura Stramonium. It was shown that, within only 2-4 hr of application of the labelled precursor, labelled metabolites can readily be detected. It was further demonstrate that de-differentiated cultures, derived from the roots by treatment with phytohirmones, are capable of metabolising [15N]tropinone to [15N]tropine and simple [15N]tropine esters but that these cultures cannot convert [15]tropinone to [15N]hyoscyamine.

Authors: Ford, Y; Ratcliffe, R; Robins, R

• Publication status: Published
• Journal: PHYTOCHEMISTRY
• Volume: 43
• Issue: 1
• Pages: 115-120
• Publication date: 1996-09-01
• DOI: 10.1016/0031-9422(96)00261-0
• ISSN: 0031-9422
• Language: English
• Keywords: phytohormones; tropanes; biosynthesis; root culture (transformed); Solanaceae; Datura stramonium; NMR (in vivo); alkaloid