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Optimization of in vitro erythropoiesis from CD34+ cord blood cells using design of experiments (DOE)

Abstract:

In vitro culture studies of hematopoietic cells (HCs) have traditionally been limited to dose-response, empirical methods which can be uninformative, misleading and suboptimal. Herein, we use design of experiments methodology to quantitatively define and optimize in vitro erythropoiesis from human cord blood CD34+ cells to enucleation. DOE was used to theoretically define the effects of 7 cytokines known to affect red cell maturation in humans. Of these, stem cell factor (SCF) and erythropoie...

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Publisher copy:
10.1016/j.bej.2011.03.014

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Biochemical Engineering Journal
Volume:
55
Issue:
3
Pages:
154-161
Publication date:
2011-08-15
DOI:
EISSN:
1369-703X
ISSN:
1369-703X
URN:
uuid:4a546fc5-c28b-4eae-b5a9-4f0195f3e314
Source identifiers:
324694
Local pid:
pubs:324694

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