Journal article
Low prevalence of 'leptospira' carriage in rodents in leptospirosis-endemic Northeastern Thailand
- Abstract:
- Leptospirosis is a neglected zoonotic disease affecting mostly the world's tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and carriage rate of pathogenic <i>Leptospira</i> in rodent reservoirs to leptospirosis incidence, we surveyed rodents, between 2011 and 2012, in four provinces in northeastern Thailand with the highest incidence rates of human leptospirosis cases. We used <i>lipL32</i> real-time PCR to detect pathogenic <i>Leptospira</i> in rodent kidneys, partial 16S rRNA gene sequencing to classify the infecting <i>Leptospira</i> species, and whole 16S rDNA sequencing to classify species of isolated <i>Leptospira</i>. Overall prevalence of <i>Leptospira</i> infection was 3.6% (18/495). Among infected rodents, <i>Bandicota</i><i>indica</i> (14.3%), <i>Rattus</i><i>exulans</i> (3.6%), and <i>R</i>. <i>rattus</i> (3.2%) had renal carriage. We identified two pathogenic <i>Leptospira</i> species: <i>L</i>. <i>interrogans</i> (<i>n</i> = 15) and <i>L</i>. <i>borgpetersenii</i> (<i>n</i> = 3). In addition, an <i>L</i>. <i>wolffii</i> (LS0914U) isolate was recovered from the urine of <i>B</i>. <i>indica</i>. <i>Leptospira</i> infection was more prevalent in low density rodent populations, such as <i>B</i>. <i>indica</i>. In contrast, there was a lower prevalence of <i>Leptospira</i> infection in high density rodent populations of <i>R</i>. <i>exulans</i> and <i>R</i>. <i>rattus</i>.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 1.6MB, Terms of use)
-
- Publisher copy:
- 10.3390/tropicalmed5040154
Authors
- Publisher:
- MDPI
- Journal:
- Tropical Medicine and Infectious Disease More from this journal
- Volume:
- 5
- Issue:
- 4
- Article number:
- 154
- Publication date:
- 2020-09-30
- Acceptance date:
- 2020-09-28
- DOI:
- EISSN:
-
2414-6366
- ISSN:
-
2414-6366
- Pmid:
-
33008058
- Language:
-
English
- Keywords:
- Pubs id:
-
1137092
- Local pid:
-
pubs:1137092
- Deposit date:
-
2021-02-10
- ARK identifier:
Terms of use
- Copyright holder:
- Krairojananan et al.
- Copyright date:
- 2020
- Rights statement:
- © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record