Journal article
Automated segmentation and alignment of mitotic nuclei for Kymograph visualisation
- Abstract:
- The mechanics of many biological processes can only be uncovered through the analysis of spatio-temporal data. Kymographs are a popular tool for visualising dynamic processes whose movements can be mapped into a single dimension, such as mitosis, or cell division. However, global movements of a cell means the region of interest (ROI) used to create the kymograph must move with each frame. Here we present an automated algorithm for the selection of dynamic ROIs to allow the construction of kymographs of proteins associated with the mitotic spindle in Drosophila embryos. We segment a dividing nucleus using a level set coupled to a shape model, which we then use to align the segmented nuclei to a common frame of reference.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 908.1KB, Terms of use)
-
- Publisher copy:
- 10.1109/ISBI.2011.5872484
Authors
Contributors
+ Engineering in Medicine and Biology Society
- Role:
- Other
+ Signal Processing Society
- Role:
- Other
+ Engineering and Physical Sciences Research Council
More from this funder
- Funding agency for:
- Li, S
- Grant:
- EP/E501605/1
- EP/E501605/1
- Publisher:
- Institute of Electrical and Electronic Engineers
- Journal:
- 2011 IEEE International Symposium on Biomedical Imaging: From Nano to Macro More from this journal
- Pages:
- 622 - 625
- Edition:
- Accepted Manuscript
- DOI:
- ISSN:
-
1945-7928
- Language:
-
English
- Subjects:
- UUID:
-
uuid:476ee223-13e4-44ab-bb11-d2e4d3288080
- Local pid:
-
ora:6008
- Deposit date:
-
2012-01-12
Terms of use
- Copyright holder:
- Institute of Electrical and Electronics Engineers (IEEE)
- Copyright date:
- 2011
- Notes:
-
© 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be
obtained for all other uses, in any current or future media, including
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