Journal article

Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde.

Abstract:: (Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation. © 2010 Wiley-VCH Verlag GmbHand Co. KGaA.

Publication status:: Published

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APA Style

Hopkinson, R., Hamed, R., Rose, N., Claridge, T., & Schofield, C. (2010). Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. Chembiochem : a European Journal of Chemical Biology, 11(4), 506–510.

MLA Style

Hopkinson, R, et al. “Monitoring the Activity of 2-Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde.” Chembiochem : a European Journal of Chemical Biology, vol. 11, no. 4, 2010, pp. 506–10.

Chicago Style

Hopkinson, R, R Hamed, N Rose, T Claridge, and C Schofield. 2010. “Monitoring the Activity of 2-Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde.” Chembiochem : a European Journal of Chemical Biology 11 (4): 506–10.
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Publisher copy:: 10.1002/cbic.200900713

Authors

+ Hopkinson, R More by this author

Role:: Author

+ Hamed, R More by this author

Role:: Author

+ Rose, N More by this author

Role:: Author

+ Claridge, T More by this author

Role:: Author

+ Schofield, C More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author

Journal:: Chembiochem : a European journal of chemical biology More from this journal
Volume:: 11
Issue:: 4
Pages:: 506-510
Publication date:: 2010-03-01
DOI:: 10.1002/cbic.200900713
EISSN:: 1439-7633
ISSN:: 1439-4227

Language:: English
Keywords:: Formaldehyde

Escherichia coli

Humans

Jumonji Domain-Containing Histone Demethylases

Magnetic Resonance Spectroscopy

Ketoglutaric Acids

Peptides
Pubs id:: pubs:46168
UUID:: uuid:474ceeb1-6fb7-413d-8f76-a3fce0ff0283
Local pid:: pubs:46168
Source identifiers:: 46168
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_474ceeb16fb7413d8f76a3fce0ff0283

Terms of use

Copyright date:: 2010

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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