CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas.

Jackson, D; Waibel, R; Weber, E; Bell, J; Stahel, R

Journal article

CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas.

Abstract:: Cell lines derived from human small cell carcinoma of the lung express high levels of a surface polypeptide termed the cluster-w4 antigen, which was previously identified as a potential target for toxin-based immunotherapy of lung cancer. We have cloned a complementary DNA encoding the cluster-w4 antigen from COS-1 fibroblasts transfected with a SW2 small cell carcinoma library, by panning with a mixture of the cluster-w4-specific monoclonal antibodies SWA11, SWA21, and SWA22. The sequence of the cluster-w4 complementary DNA encodes an unusually short (80-amino acid) protein identical to that recently reported for the leukocyte activation molecule CD24 except for a single valine-alanine substitution due to a single-base polymorphism within the region of the gene coding for the extracellular domain. Biochemical analyses of the cloned cluster-w4 antigen confirmed both the presence of the phosphatidylinositol tail and the extensive glycosylation reported for the CD24 molecule. Furthermore, the cloned cluster-w4 antigen expressed on COS cells was shown to react with a comprehensive panel of CD24-specific monoclonal antibodies, as assessed by indirect immunofluorescence staining. Northern blot hybridization indicated the presence of several transcript sizes for the cluster-w4 antigen that were greatly overexpressed in small cell carcinoma cell lines, compared with normal hemopoietic cells and CD24-positive cell lines. Southern blot hybridization of restriction digests of genomic DNA identified a complex pattern of bands consistent with either a complex gene structure containing many exons or the presence of a family of closely related genes.

Publication status:: Published

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APA Style

Jackson, D., Waibel, R., Weber, E., Bell, J., & Stahel, R. (1992). CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas. Cancer Research, 52(19), 5264–5270.

MLA Style

Jackson, D., et al. “CD24, a Signal-Transducing Molecule Expressed on Human B Cells, Is a Major Surface Antigen on Small Cell Lung Carcinomas.” Cancer Research, vol. 52, no. 19, 1992, pp. 5264–70.

Chicago Style

Jackson, D, R Waibel, E Weber, J Bell, and R Stahel. 1992. “CD24, a Signal-Transducing Molecule Expressed on Human B Cells, Is a Major Surface Antigen on Small Cell Lung Carcinomas.” Cancer Research 52 (19): 5264–70.
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