Automated solid-phase extraction for determination of amodiaquine, chloroquine and metabolites in capillary blood on sampling paper by liquid chromatography

Journal article

A bioanalytical method using solid-phase extraction and high-performance liquid chromatography (LC) has been developed and validated for the determination of amodiaquine, chloroquine and their metabolites in 100 μL blood applied to sampling paper. The intra-assay precision for all analytes was < 5% at 2000 nM, < 7% at 750 nM and < 10% at 100 nM. The inter-assay precision for all analytes was < 4% at 2000 nM, < 7% at 750 nM and < 12% at 100 nM. The lower limit of quantitation was 100 nM for all analytes. The limit of detection was 30 nM in 100 μL venous blood opplied to sampling paper.

Authors: Lindegardh, N; Forslund, M; Green, MD; Kaneko, A; Bergqvist, Y

• Publication status: Published
• Journal: CHROMATOGRAPHIA
• Volume: 55
• Issue: 1-2
• Pages: 5-12
• Publication date: 2002-01-01
• DOI: 10.1007/BF02492307
• EISSN: 1612-1112
• ISSN: 0009-5893
• Language: English
• Keywords: column liquid chromatography; capillary blood; ion-pair chromatography; solid-phase extraction; antimalarial drugs