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The CRAPome: A contaminant repository for affinity purification-mass spectrometry data

Abstract:

Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background contaminants (for example, proteins that interact with the solid-phase support, affinity reagent or epitope tag) is a challenging task. The standard approach is to identify nonspecific interaction...

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Publisher copy:
10.1038/nmeth.2557

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Journal:
Nature Methods
Volume:
10
Issue:
8
Pages:
730-736
Publication date:
2013-08-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:45d95c03-f7b8-4091-9091-817b0f11bc9d
Source identifiers:
419227
Local pid:
pubs:419227
Language:
English

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