Characterization of anti-HIV-1 neutralizing and binding antibodies in chronic HIV-1 subtype C infection.

Journal article

Neutralizing (nAbs) and high affinity binding antibodies may be critical for an efficacious HIV-1 vaccine. We characterized virus-specific nAbs and binding antibody responses over 21 months in eight HIV-1 subtype C chronically infected individuals with heterogeneous rates of disease progression. Autologous nAb titers of study exit plasma against study entry viruses were significantly higher than contemporaneous responses at study entry (p=0.002) and exit (p=0.01). NAb breadth and potencies against subtype C viruses were significantly higher than for subtype A (p=0.03 and p=0.01) or B viruses (p=0.03; p=0.05) respectively. Gp41-IgG binding affinity was higher than gp120-IgG (p=0.0002). IgG-FcγR1 affinity was significantly higher than FcγRIIIa (p<0.005) at study entry and FcγRIIb (p<0.05) or FcγRIIIa (p<0.005) at study exit. Evolving IgG binding suggests alteration of immune function mediated by binding antibodies. Evolution of nAbs was a potential marker of HIV-1 disease progression.

Authors: Archary, D; Rong, R; Gordon, M; Boliar, S; Madiga, M

• Journal: Virology
• Volume: 433
• Issue: 2
• Pages: 410-420
• Publication date: 2012-11-01
• DOI: 10.1016/j.virol.2012.08.033
• EISSN: 1096-0341
• ISSN: 0042-6822
• Language: English
• Keywords: Female; Humans; HIV Infections; Young Adult; HIV Antibodies; Retrospective Studies; Immunoglobulin G; Disease Progression; HIV Antigens; Antigenic Variation; Chronic Disease; HIV-1; Middle Aged; Adult; Male; Viral Load; Epitope Mapping; CD4 Lymphocyte Count; Antibodies, Neutralizing; Antibody Specificity; Receptors, IgG