Flow cytometric measurement of intracellular Th1 and Th2 cytokine production by human villous and extravillous cytotrophoblast.

Journal article

A wide variety of cytokines are present at the maternal-fetal interface, but the extreme cellular complexity of the placenta has made it difficult to determine which cytokines are produced by which cells. Hence novel flow cytometric methods have been applied to determine intracellular cytokine production by specific cell-types in placental cell suspensions. Cell suspensions were prepared from first and third trimester chorionic villi and third trimester amniochorion by enzymatic digestion and...

Expand abstract

Authors: Sacks, G; Clover, L; Bainbridge, DR; Redman, C; Sargent, I

• Publication status: Published
• Journal: Placenta
• Volume: 22
• Issue: 6
• Pages: 550-559
• Publication date: 2001-07-01
• DOI: 10.1053/plac.2001.0686
• EISSN: 1532-3102
• ISSN: 0143-4004
• Language: English
• Keywords: Placenta; Chorion; Humans; Pregnancy Trimester, Third; Interferon-gamma; Pregnancy Trimester, First; RNA, Messenger; Cell Separation; Keratins; Centrifugation, Density Gradient; Female; Chorionic Villi; Trophoblasts; Cytokines; Receptor, erbB-2; Receptor, Epidermal Growth Factor; Histocompatibility Antigens Class I; Interleukin-4; Flow Cytometry; Pregnancy; Tumor Necrosis Factor-alpha; Interleukin-12; Interleukin-10