Journal article
The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core
- Abstract:
- Redox regulated effector systems that counteract oxidative stress are essential for all forms of life. Here, we uncover a new paradigm for sensing oxidative stress centred on the hydrophobic core of a sensor protein. RsrA is an archetypal zinc-binding anti-sigma factor that responds to disulfide stress in the cytoplasm of Actinobacteria. We show that RsrA utilizes its hydrophobic core to bind the sigma factor σR preventing its association with RNA polymerase, and that zinc plays a central role in maintaining this high-affinity complex. Oxidation of RsrA is limited by the rate of zinc release, which weakens the RsrA-σR complex by accelerating its dissociation. The subsequent trigger disulfide, formed between specific combinations of RsrA’s three zinc-binding cysteines, precipitates structural collapse to a compact state where all σR-binding residues are sequestered back into its hydrophobic core, releasing σR to activate transcription of anti-oxidant genes.
- Publication status:
- Accepted
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.3MB, Terms of use)
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- Publisher copy:
- 10.1038/ncomms12194
Authors
- Publisher:
- Nature Publishing Group: Nature Communications
- Journal:
- Nature Communications More from this journal
- Volume:
- 7
- Issue:
- 12194
- Pages:
- 1-14
- Publication date:
- 2016-07-19
- Acceptance date:
- 2016-06-08
- DOI:
- ISSN:
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2041-1723
- Language:
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English
- Pubs id:
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pubs:631767
- UUID:
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uuid:44e5ab04-64e6-43a2-91aa-be0fe3bc173f
- Local pid:
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pubs:631767
- Source identifiers:
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631767
- Deposit date:
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2016-07-04
- ARK identifier:
Terms of use
- Copyright holder:
- Rajasekar et al
- Copyright date:
- 2016
- Notes:
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© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license hol
This is the publisher's version of the article. The final version is available online from Nature Publishing Group at: https://doi.org/10.1038/ncomms12194
- Licence:
- CC Attribution (CC BY)
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