Hypoxia-inducible expression of tumor-associated carbonic anhydrases.

Journal article

The transcriptional complex hypoxia-inducible factor-1 (HIF-1) has emerged as an important mediator of gene expression patterns in tumors, although the range of responding genes is still incompletely defined. Here we show that the tumor-associated carbonic anhydrases (CAs) are tightly regulated by this system. Both CA9 and CA12 were strongly induced by hypoxia in a range of tumor cell lines. In renal carcinoma cells that are defective for the von Hippel-Lindau (VHL) tumor suppressor, up-regul...

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Authors: Wykoff, C; Beasley, N; Watson, P; Turner, K; Pastorek, J

• Publication status: Published
• Journal: Cancer research
• Volume: 60
• Issue: 24
• Pages: 7075-7083
• Publication date: 2000-12-01
• EISSN: 1538-7445
• ISSN: 0008-5472
• Language: English
• Keywords: Vascular Endothelial Growth Factors; Carcinoma; Models, Genetic; Hypoxia-Inducible Factor 1; DNA-Binding Proteins; Kidney Neoplasms; Transcription Factors; In Situ Hybridization; Endothelial Growth Factors; RNA; Carbonic Anhydrases; Nitroimidazoles; Immunohistochemistry; Promoter Regions, Genetic; Tumor Cells, Cultured; Necrosis; Skin Neoplasms; Lymphokines; Hypoxia-Inducible Factor 1, alpha Subunit; Nuclear Proteins; Urinary Bladder Neoplasms; Immunoblotting; RNA, Messenger; Radiation-Sensitizing Agents; Up-Regulation; Humans; Anoxia; Genes, Reporter; Blotting, Western; Carcinoma, Renal Cell; Oxygen; Plasmids; Vascular Endothelial Growth Factor A