Journal article
AQP4 antibody assay sensitivity comparison in the era of the 2015 diagnostic criteria for NMOSD
- Abstract:
- We have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were referred from 23 centers. Clinical details and magnetic imaging were reviewed and used to apply the 2015 IPND diagnostic criteria. In addition, 101 age- and sex-matched patients with multiple sclerosis were referred. Other inflammatory disease (n = 49) and healthy controls (n = 103) were also recruited. Samples from all participants were tested using tissue-based indirect immunofluorescence assays and a subset were tested using four additional ELISA and cell-based assays. Antibodies to myelin oligodendrocyte glycoprotein (MOG) were also assayed. All aquaporin-4 antibody assays proved to be highly specific. Sensitivities ranged from 60 to 94%, with cell-based assays having the highest sensitivity. Antibodies to MOG were detected in 8/79 (10%) of the residual suspected cases of NMOSD. Under the 2015 IPND diagnostic criteria for NMOSD, cell-based assays for aquaporin-4 are sensitive and highly specific, performing better than tissue-based and ELISA assays. A fixed cell-based assay showed near-identical results to a live-cell based assay. Antibodies to MOG account for only a small number of suspected cases.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 435.3KB, Terms of use)
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- Publisher copy:
- 10.3389/fneur.2019.01028
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Neurology More from this journal
- Volume:
- 10
- Article number:
- 1028
- Publication date:
- 2019-10-04
- Acceptance date:
- 2019-09-10
- DOI:
- EISSN:
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1664-2295
- Pmid:
-
31636597
- Language:
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English
- Keywords:
- Pubs id:
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pubs:1065838
- UUID:
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uuid:440098bb-8725-464d-badb-f82980c778d7
- Local pid:
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pubs:1065838
- Source identifiers:
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1065838
- Deposit date:
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2019-12-18
- ARK identifier:
Terms of use
- Copyright holder:
- Prain et al
- Copyright date:
- 2019
- Notes:
- Copyright © 2019 Prain, Woodhall, Vincent, Ramanathan, Barnett, Bundell, Parratt, Silvestrini, Bukhari, The Australian and New Zealand NMO Collaboration, Brilot, Waters and Broadley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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