Journal article icon

Journal article

Coarse-grained simulations of DNA overstretching.

Abstract:
We use a recently developed coarse-grained model to simulate the overstretching of duplex DNA. Overstretching at 23 degrees C occurs at 74 pN in the model, about 6-7 pN higher than the experimental value at equivalent salt conditions. Furthermore, the model reproduces the temperature dependence of the overstretching force well. The mechanism of overstretching is always force-induced melting by unpeeling from the free ends. That we never see S-DNA (overstretched duplex DNA), even though there is clear experimental evidence for this mode of overstretching under certain conditions, suggests that S-DNA is not simply an unstacked but hydrogen-bonded duplex, but instead probably has a more exotic structure.
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Publisher copy:
10.1063/1.4792252

Authors


More by this author
Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author


Publisher:
American Institute of Physics
Journal:
Journal of Chemical Physics More from this journal
Volume:
138
Issue:
8
Article number:
085101
Publication date:
2013-02-28
DOI:
EISSN:
1089-7690
ISSN:
0021-9606


Language:
English
Keywords:
Pubs id:
pubs:352667
UUID:
uuid:430db1f3-25d6-4548-aaba-6abe5b029623
Local pid:
pubs:352667
Source identifiers:
352667
Deposit date:
2013-09-26

Terms of use



Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP