Whither metabolic flux analysis in plants?

Journal article

Abstract:: Stable isotope labelling experiments provide many opportunities for probing metabolic pathways. One goal of such experiments is to define the metabolic phenotype of an organism in terms of the fluxes supported by the entire metabolic network. Metabolic flux analysis (MFA) is used routinely in prokaryotes, but its application to plants is more challenging. Here we examine the status of MFA in plants, highlighting difficulties that hinder wider exploitation of the technique. We conclude that simulation of network fluxes using constraints-based modelling offers a more versatile approach for exploring the capabilities of a network, and that MFA might be best used to probe the interesting features that simulation reveals.

Files:: Kruger_and_Ratcliffe_2021_whither_metabolic_flux.pdf

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Language:: English
Keywords:: FFR

flux balance analysis

metabolic flux analysis

metabolic phenotype

metabolic network

steady-state analysis

non-stationary analysis
Pubs id:: 1193675
Local pid:: pubs:1193675
Deposit date:: 2021-09-06
ARK identifier:: ark:/29072/ora_42e3220172a1481faf1009d38d63e812

Copyright holder:: Kruger and Ratcliffe
Rights statement:: © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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