Journal article

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors

Abstract:: In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. © Springer Science+Business Media New York 2014.

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APA Style

Bird, L., Rada, H., Flanagan, J., Diprose, J., Gilbert, R., & Owens, R. (2014). Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. Methods in Molecular Biology, 1116, 209–234.

MLA Style

Bird, L., et al. “Application of In-Fusion™ Cloning for the Parallel Construction of E. Coli Expression Vectors.” Methods in Molecular Biology, vol. 1116, Humana Press Inc., 2014, pp. 209–34.

Chicago Style

Bird, L, H Rada, J Flanagan, J Diprose, R Gilbert, and R Owens. 2014. “Application of In-Fusion™ Cloning for the Parallel Construction of E. Coli Expression Vectors.” Methods in Molecular Biology 1116: 209–34.
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Publisher copy:: 10.1007/978-1-62703-764-8_15

Authors

+ Bird, L More by this author

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+ Rada, H More by this author

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+ Flanagan, J More by this author

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+ Diprose, J More by this author

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+ Gilbert, R More by this author

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Publisher:: Humana Press Inc.
Journal:: Methods in Molecular Biology More from this journal
Volume:: 1116
Pages:: 209-234
Publication date:: 2014-01-01
DOI:: 10.1007/978-1-62703-764-8_15
ISSN:: 1064-3745

Language:: English
Keywords:: Periplasmic secretion

Single-stranded annealing

In-Fusion™ enzyme

PCR cloning

Co-expression

Fusion tags

Escherichia coli
Pubs id:: pubs:476991
UUID:: uuid:41bdb6f4-dc42-4f21-a8ba-00be8bccf3aa
Local pid:: pubs:476991
Source identifiers:: 476991
Deposit date:: 2014-08-06

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Copyright date:: 2014

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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