Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1.

Journal article

The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA.

Authors: Turner, M; Chantry, D; Feldmann, M

• Publication status: Published
• Journal: Biochemical and biophysical research communications
• Volume: 156
• Issue: 2
• Pages: 830-839
• Publication date: 1988-10-01
• DOI: 10.1016/s0006-291x(88)80919-7
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Amanitins; Gene Expression Regulation; Interleukin-1; Transcription, Genetic; Tumor Cells, Cultured; Half-Life; RNA Polymerase II; Female; Lipopolysaccharides; Kinetics; Cycloheximide; Leukemia, Monocytic, Acute; Humans; RNA, Messenger; Tetradecanoylphorbol Acetate