Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers

Journal article

Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.

Authors: Roberts, RF; Bengoa-Vergniory, N; Alegre-Abarrategui, J

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Springer Publisher's website
• Series: Methods in Molecular Biology
• Series number: 1948
• Article number: 69-76
• Publication date: 2019-02-16
• Acceptance date: 2019-02-16
• DOI: 10.1007/978-1-4939-9124-2_7
• EISSN: 1940-6029
• ISSN: 1064-3745
• Pmid: 30771171
• EISBN: 9781493991242
• ISBN: 9781493991235
• Language: English
• Keywords: pathology; alpha-synuclein; Parkinson's disease; proximity ligation assay; oligomers; synucleinopathy; FFR; aggregates