Book section
Alpha-synuclein proximity ligation assay (AS-PLA) in brain sections to probe for alpha-synuclein oligomers
- Abstract:
- Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 237.9KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-4939-9124-2_7
Authors
- Publisher:
- Springer
- Host title:
- Alpha-Synuclein
- Pages:
- 69-76
- Article number:
- 69-76
- Chapter number:
- 7
- Series:
- Methods in Molecular Biology
- Series number:
- 1948
- Publication date:
- 2019-02-16
- Acceptance date:
- 2019-02-16
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Pmid:
-
30771171
- EISBN:
- 9781493991242
- ISBN:
- 9781493991235
- Language:
-
English
- Keywords:
- Pubs id:
-
975204
- Local pid:
-
pubs:975204
- Deposit date:
-
2020-01-29
- ARK identifier:
Terms of use
- Copyright holder:
- Springer
- Copyright date:
- 2019
- Rights statement:
- © Springer Science+Business Media, LLC, part of Springer Nature 2019.
- Notes:
- This is the accepted manuscript version of the chapter. The final version is available online from Springer at: https://doi.org/10.1007/978-1-4939-9124-2_7
If you are the owner of this record, you can report an update to it here: Report update to this record