The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers.

Journal article

Two types of stem cells are currently defined in small intestinal crypts: cycling crypt base columnar (CBC) cells and quiescent '+4' cells. Here, we combine transcriptomics with proteomics to define a definitive molecular signature for Lgr5(+) CBC cells. Transcriptional profiling of FACS-sorted Lgr5(+) stem cells and their daughters using two microarray platforms revealed an mRNA stem cell signature of 384 unique genes. Quantitative mass spectrometry on the same cell populations identified 27...

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Authors: Muñoz, J; Stange, D; Schepers, A; van de Wetering, M; Koo, B

• Publication status: Published
• Journal: EMBO journal
• Volume: 31
• Issue: 14
• Pages: 3079-3091
• Publication date: 2012-07-01
• DOI: 10.1038/emboj.2012.166
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Mice, Transgenic; Animals; Mice; Gene Expression Profiling; Receptors, G-Protein-Coupled; Calcium-Binding Proteins; Antigens, Differentiation; Gene Expression Regulation; Stem Cells; RNA, Messenger; Oligonucleotide Array Sequence Analysis; Intestines